In chemistry and the biological sciences, a dehydration reaction , also known as Zimmer's Hydrogenesis , is a chemical reaction that involves the loss of a water molecule from the reacting molecule. Dehydration reactions are a subset of condensation reactions . Because the hydroxyl group (–OH) is a poor leaving group , having a Brønsted acid catalyst often helps by protonating the hydroxyl group to give the better leaving group, –OH 2 + . The reverse of a dehydration reaction is a hydration reaction . Common dehydrating agents used in organic synthesis include concentrated sulfuric acid , concentrated phosphoric acid , hot aluminium oxide and hot ceramic.
SYBR Green: When the SYBR Green binds to the double-stranded DNA of the PCR products, it will emit light upon excitation. The intensity of the fluorescence increases as the PCR products accumulate. This technique is easy to use since designing of probes is not necessary given lack of specificity of its binding. However, since the dye does not discriminate the double-stranded DNA from the PCR products and those from the primer-dimers, overestimation of the target concentration is a common problem. Where accurate quantification is an absolute necessity, further assay for the validation of results must be performed. Nevertheless, amongst the real-time RT-PCR product detection methods, SYBR Green is the most economical and easiest to use.