Neural injections of Bromodeoxyuridine (BrdU) were applied to males of both groups to test for neurogenesis . Analysis showed that testosterone and dihydrotestosterone regulated adult hippocampal neurogenesis (AHN). Adult hippocampal neurogenesis was regulated through the androgen receptor in the wild-type male rats, but not in the TMF male rats. To further test the role of activated androgen receptors on AHN, flutamide , an antiandrogen drug that competes with testosterone and dihydrotestosterone for androgen receptors , and dihydrotestosterone were administered to normal male rats. Dihydrotestosterone increased the number of BrdU cells, while flutamide inhibited these cells.
Enzalutamide has greater affinity to AR than Bicalutamide does in a competition assay with 16β-[ 18 F]fluoro-5α-DHT (18-FDHT) in castration-resistant LNCaP/AR cells (AR-overexpressing). While Enzalutamide shows no agonism in LNCaP/AR prostate cells. Enzalutamide antagonizes induction of prostate-specific antigen (PSA) and transmembrane serine protease 2 (TMPRSS2), combination with the synthetic androgen R1881 in parental LNCaP cells. Enzalutamide could inhibit the transcriptional activity of a mutant AR protein (W741C, mutation of Trp 741 to Cys).  Enzalutamide also prevents nuclear translocation and co-activator recruitment of the ligand-receptor complex. 
Hyperandrogenism is a condition in women in which androgen levels are excessively and abnormally high.  It is commonly seen in women with PCOS, and also occurs in women with intersex conditions like congenital adrenal hyperplasia .  Hyperandrogenism is associated with virilization – that is, the development of masculine secondary sexual characteristics like male-pattern facial and body hair growth (or hirsutism), voice deepening , increased muscle mass and strength , and broadening of the shoulders, among others.  Androgen-dependent skin and hair conditions like acne and androgenic alopecia may also occur in hyperandrogenism, and menstrual disturbances , like amenorrhea , are commonly seen.  Although antiandrogens do not treat the underlying cause of hyperandrogenism (., PCOS), they are able to prevent and reverse its manifestation and effects.  As with androgen-dependent skin and hair conditions, the most commonly used antiandrogens in the treatment of hyperandrogenism in women are cyproterone acetate and spironolactone.  Other antiandrogens, like bicalutamide, may be used alternatively.